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Influence de la rugosité de surfaces Ti-6Al-4V obtenues par fabrication additive SLM sur l'ostéointégration : Évaluation in vitro et in silico

Abstract : Osseointegration defines the connection between the bone and the implant and it ensures the proper functioning of the implant. Such a connection is governed, among others, by the surface properties of the implant, which influence the cells response. Surface roughness can significantly modify different cellular processes (i.e. migration, adhesion, proliferation…) occurring during growth. Additive manufacturing (FA) "Selective Laser Melting" (SLM) is an innovative process bringing many advantages in the biomedical field. However, samples obtained by FA SLM exhibit a very rough surface which affects cellular response and osseointegration. Therefore, the use of this process for a biomedical application requires the understanding of the effects of roughness on biocompatibility. This thesis presents an experimental and numerical methodology allowing to evaluate the influence of roughness of surfaces obtained by FA SLM on the cellular behavior. The roughness and the wettability of raw SLM surfaces were characterized by microscopic, profilometer and wettability observations. In addition, several surface treatments (i.e. chemical etching and sandblasting) have been applied in order to seek an improvement in the biocompatibility of the surfaces. Biological cytocompatibility tests as well as analysis of cell morphology have validated the biocompatibility of raw SLM surfaces and modified surfaces. However, the tests did not show a preferential treatment of the surfaces which ensures a significant improvement in biocompatibility. In parallel, a discrete numerical model of cell migration and proliferation has been developed to quantify the influence of roughness at the microscopic scale on cellular activities. The results have shown an important role of topographic parameters on migration as well as cell proliferation.
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Submitted on : Friday, December 10, 2021 - 10:07:51 AM
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Long-term archiving on: : Friday, March 11, 2022 - 6:35:48 PM

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  • HAL Id : tel-03474194, version 1

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Thomas Thenard. Influence de la rugosité de surfaces Ti-6Al-4V obtenues par fabrication additive SLM sur l'ostéointégration : Évaluation in vitro et in silico. Biomécanique [physics.med-ph]. HESAM Université, 2021. Français. ⟨NNT : 2021HESAE009⟩. ⟨tel-03474194⟩

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