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Compréhension et contrôle de la morphologie des champignons filamenteux en culture liquide

Abstract : Biotechnology has for many years operated a large collection of microorganisms in food and health sectors. Today, this field fits perfectly into the ecological transition of chemical and environmental sectors, by designing high-performance processes to replace petroleum products. Filamentous fungi represent important organisms in industrial biotechnologies, widely used for the production of metabolites with strong commercial interests such as antibiotics, enzymes or organic acids. Their implementation in submerged environment is continually attracting new research to overcome issues associated with their behavior. Indeed, this mode of cultivation generates different fungal morphologies, mainly dependent on their intrinsic biological properties but also on physico-chemical conditions within their environment. This characteristic of filamentous fungi very often influences their implementation and their productivity.This thesis proposes an original study to identify and control the underlying phenomena that influence fungal morphogenesis. To this end, we propose research combining fundamental and applied studies on several strains of strong industrial interest. We have investigated and developed various pioneering techniques for the control of fungal morphology. Microparticles implementation and development of a microfluidic encapsulation technique allowed us to confirm and deepen the aggregation mechanisms of fungal conidia. On the other hand, the addition of mineral salts in the culture medium prevented hyphae aggregation. To validate an extrapolation of these results towards an industrial process, these tools were also implemented during the first steps of scale-up. These works bring results and perspectives in the control of bioprocesses involving filamentous fungi.
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  • HAL Id : tel-03510230, version 1

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Marion Drougard. Compréhension et contrôle de la morphologie des champignons filamenteux en culture liquide. Génie des procédés. Institut agronomique, vétérinaire et forestier de France, 2018. Français. ⟨NNT : 2018IAVF0019⟩. ⟨tel-03510230⟩

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