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Etude et modélisation de la fermentation IBE en biofilm

Abstract : For many years, biotechnologies have been employed to design numerous consumers commodities from renewable resources. Those technologies allow to synthetize industrial interest molecules from agricultural commodities. The bacteria Clostridium beijerinckii can consume hexoses to produce two platform chemicals : isopropanol and butanol. However, this bacterium suffers from an inhibition caused by butanol accumulation which causes low productivities in batch fermentation. New continuous fermentation strategies have thus been developed. Bacteria were immobilized on porous support to maintain high cellular concentration and therefore enhance the process productivity. Numerous process parameters must be optimized and understood to maximize the process productivity and ensure the scale-up efficiency. The physicochemical properties of the solid support as well as the hydrodynamic conditions can influence the biofilm development and thus the productivity of the developed process. Consequently, this PhD research is focused on the development of numerical and experimental tools which allow to better understand these phenomena . Kinetic models were used to describe the biofilm development and allowed the simulation of the process performances while taking cells attachment and detachment from biofilm into account. Moreover, new methodologies using flow cytometry and confocal microscopy were developed to characterize biofilm cells viability. Those methodologies are powerful to measure the immobilized cells viability and to describe active cells accumulation within the solid support used during time. The use of flow cytometry to characterize biofilm cells viability also helped to consider new strategies for process control and operation.
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Submitted on : Thursday, January 27, 2022 - 1:21:08 PM
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  • HAL Id : tel-03545640, version 1

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Maxime Carrié. Etude et modélisation de la fermentation IBE en biofilm. Biotechnologies. Université Paris-Saclay, 2021. Français. ⟨NNT : 2021UPASB053⟩. ⟨tel-03545640⟩

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