Skip to Main content Skip to Navigation
Theses

Spatio-temporal transcriptome analysis of surface-associated multicellular assemblages of Bacillus subtilis

Abstract : Microbial cells are frequently found associated to various surfaces, forming complex multicellular assemblages, known as biofilms. Despite some beneficial effects provided by these structures, they are often involved in human chronic infections. An undomesticated Bacillus subtilis strain (NDmed), lately isolated from a hospital, has been found to form highly structured immersed biofilms hyper-resistant to biocide action and able to protect pathogens when associated with them. Thus, understanding how these surface bounded communities are formed is crucial for their control. Bacillus subtilis biofilm studies, mainly on colony and pellicle, had allowed to identify several genes involved in the formation and the regulation of 3D structures, but their involvement in the submerged biofilm model remained unclear. In order to better understand the molecular strategies taking place during biofilm development, we have performed genetic comparative studies using different approaches, including the swarming model, temporal and spatial transcriptome profiling, identification and construction of target mutants and 4D CLSM coupled with the use of fluorescent transcriptional fusions. Data obtained from RNA-seq analysis gave a global view on the different genetic expression profiles for each of the local spatial surface-associated population. Comparison between these transcriptome profiles indicated similarities and differences exhibited between the distinct spatial surface compartments. Moreover, in situ spatio-temporal visualization of expression of several genes at single cell level revealed undescribed patterns suggesting new mechanisms of regulation in these structured communities.
Document type :
Theses
Complete list of metadata

https://pastel.archives-ouvertes.fr/tel-03549216
Contributor : ABES STAR :  Contact
Submitted on : Monday, January 31, 2022 - 12:22:15 PM
Last modification on : Friday, August 5, 2022 - 2:38:11 PM
Long-term archiving on: : Sunday, May 1, 2022 - 7:12:37 PM

File

106171_DERGHAM_2021_archivage....
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-03549216, version 1

Citation

Yasmine Dergham. Spatio-temporal transcriptome analysis of surface-associated multicellular assemblages of Bacillus subtilis. Microbiology and Parasitology. Université Paris-Saclay, 2021. English. ⟨NNT : 2021UPASB064⟩. ⟨tel-03549216⟩

Share

Metrics

Record views

98

Files downloads

93