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Theses

Étude des interactions entre des substrats microstructurés et des cellules endothéliales pour le développement d’un biocapteur plasmonique pour le diagnostic précoce de conditions pathologiques

Abstract : The integrity of the vascular endothelium depends on the cohesion of the endothelial cells that compose the cell monolayer. This cohesion is reinforced by endothelial junctions mainly composed of VE-cadherins. Quantifying the integrity of this cell layer is particularly important in the study and diagnosis of pathological conditions associated with inflammatory stress and exposure to bacterial toxins such as in the case of sepsis.Based on the association of these inflammatory conditions with a loss of integrity of the vascular endothelium, we suggest in this project the development and validation of next-generation plasmonic biochips, with the aim of generating models of normalized cell layers in vitro, in order to quantify, in real time and without labeling, the integrity of the endothelial barrier.In this thesis, an in vitro cell monolayer model consisting of 3 hexagons was fabricated. This is a first proof of concept which confirms that the strategy of geometrically controlling the confinement of cells and then their connectivity by optimizing the parameters of self-assembly and adhesion, makes it possible to induce a functional cell monolayer as a standardized endothelial model. in vitro. This is a first brick for the production, ultimately, of a more complete endothelial tissue made up of more hexagons, for the development of new generation plasmonic biochips for the early diagnosis of pathological conditions such as sepsis.This doctorate is carried out in joint supervision between the University of Sherbrooke and the University of Paris-Saclay. The manufacture of gold substrates and chrome/quartz masks for soft photolithography techniques as well as image processing are carried out at the Interdisciplinary Institute for Technological Innovation (3IT), expertise in plasmonic imaging is provided by the Charles Fabry laboratory (LCF) at the Institut d'Optique Graduate School in France (IOGS), and the surface chemistry, microfabrication, cell cultures and biochip validation experiments are carried out at the pharmacology department in Sherbrooke (IPS).
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https://pastel.archives-ouvertes.fr/tel-03665218
Contributor : Abes Star :  Contact
Submitted on : Wednesday, May 11, 2022 - 3:37:16 PM
Last modification on : Tuesday, May 24, 2022 - 3:37:30 AM

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  • HAL Id : tel-03665218, version 1

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Zhor Khadir. Étude des interactions entre des substrats microstructurés et des cellules endothéliales pour le développement d’un biocapteur plasmonique pour le diagnostic précoce de conditions pathologiques. Optique [physics.optics]. Université Paris-Saclay; Université de Sherbrooke (Québec, Canada), 2022. Français. ⟨NNT : 2022UPAST035⟩. ⟨tel-03665218⟩

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