Skip to Main content Skip to Navigation
Theses

Study of C. maltaromaticum CNCM I-3298 to improve performance of biological time-temperature integrators and gain more insight into lactic acid bacteria cryoresistance

Abstract : C. maltaromaticum was selected in the development of biological time-temperature integrators (TTI), mainly because it is a lactic acid bacterium able to grow within the storage temperature range of perishable foods and it exhibits exceptional cryoresistance. In other lactic acid bacteria, freezing can lead to degradation of their technological functions (viability and acidifying activity), making their use at industrial scale difficult. This is particularly the case for Lactobacillus delbrueckii subsp. bulgaricus, an industrially relevant dairy starter. This work was structured around two objectives: (i) design strategies for the production of C. maltaromaticum concentrates with physiological responses of interest for TTIs and (ii) use C. maltaromaticum as a model strain of lactic acid bacteria cryoresistance, to help identify markers of cryoresistance.To address the first objective, concentrates were produced under different culture conditions, and theirgrowth, metabolite production and final physiological state were evaluated. This allowed the development of two models; one to optimize the culture of C. maltaromaticum and the other to produce concentrates for use in a TTI following a specific shelf life. To addressthe second objective, Fourier transform infrared (FTIR) spectroscopy and other analytical approaches were used to characterize fresh and thawed samples of C. maltaromaticum and two L. bulgaricus strains with varying cryoresistances. The maintenance upon freezing of a highly fluid membrane, of protein conformation and of the infrared band intensities associated to cell wall components were identified as cellular markers of LABcryoresistance
Document type :
Theses
Complete list of metadata

https://pastel.archives-ouvertes.fr/tel-03671246
Contributor : ABES STAR :  Contact
Submitted on : Wednesday, May 18, 2022 - 11:11:12 AM
Last modification on : Friday, August 5, 2022 - 2:41:13 PM

File

97315_GIRARDEAU_2021_archivage...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-03671246, version 1

Citation

Amélie Girardeau. Study of C. maltaromaticum CNCM I-3298 to improve performance of biological time-temperature integrators and gain more insight into lactic acid bacteria cryoresistance. Microbiology and Parasitology. Université Paris-Saclay, 2021. English. ⟨NNT : 2021UPASB068⟩. ⟨tel-03671246⟩

Share

Metrics

Record views

66

Files downloads

8