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Assemblage systématique de capteurs électrochimiques d'antigène spécifique de la prostate à base de phtalocyanines Co(II) asymétriques, de points quantiques graphitiques et d'un aptamère

Abstract : In this PhD work, electrochemical sensors based on aptamers, called aptasensors, were designed and optimised for prostate specific antigen (PSA) diagnosis. For this purpose, first innovative nanocomposites that were either undoped or doped graphene-based quantum dots, and gold nanoparticles; as well as asymmetric metallophthalocyanines (MPcs) were synthesised and characterised physico-chemically by various classical analytical methods (in solid state or in solution) and an innovative coupling of electrokinetic separation with mass spectrometry. This characterisation has allowed to improve the synthesis protocols of various graphene quantum dots, in order to achieve the expected properties of these nano-objects, in terms of size, charge density, colloidal stability, and to help for their subsequent functionalisation or the generation of specific interaction with electrode surfaces. These nanocomposites were then functionalized with aptamers and/or MPcs, and the combinations were characterized by the previous methods. The functionalized nanocomposites were deposited on the surface of glassy carbon electrodes by either adsorption, self-assembly, electrochemical grafting or click chemistry to generate electrochemical aptamers for PSA. Quantification was performed by electrochemical impedance spectroscopy and differential pulse voltammetry. These sensors showed good sensitivity, selectivity and repeatability, with detection limits in the range of 1.2- to 2.0 pM in model buffers and spiked serum samples, compatible with early diagnosis of prostate cancer. In addition, the synergistic effects of AuNPs and CoPcs-based quantum dots were demonstrated using electrochemical methods.
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https://pastel.archives-ouvertes.fr/tel-03727523
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Submitted on : Tuesday, July 19, 2022 - 12:14:12 PM
Last modification on : Wednesday, July 20, 2022 - 3:46:14 AM

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Siphesihle Nxele. Assemblage systématique de capteurs électrochimiques d'antigène spécifique de la prostate à base de phtalocyanines Co(II) asymétriques, de points quantiques graphitiques et d'un aptamère. Chimie organique. Université Paris sciences et lettres; Rhodes university (Grahamstown, Afrique du Sud), 2021. Français. ⟨NNT : 2021UPSLC028⟩. ⟨tel-03727523⟩

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